BREEDING: PROGESTERONE
However, tests do exist to determine a peak period for successful mating. One of the most reliable is a test which monitors progesterone levels in the blood. Progesterone is a male hormone found in every female, to lessor amounts than in males.
A bitch about to be bred will experience a significant drop in progesterone levels just as she begins to ovulate. Knowing the drop has occurred alerts most breeders that active breeding should begin.
Written by Ron Lueth, Pet Guardian Angels of America